fastp -i  R1.fq.gz -I R2.fq.gz -o R1.clean.fastq -O R2.clean.fastq

–json fastp.json –html fastp.html

–qualified_quality_phred 15

–unqualified_percent_limit 40 –length_required 25

–detect_adapter_for_pe –cut_tail –cut_window_size 5

–cut_mean_quality 20 –detect_adapter_for_pe

–overrepresentation_analysis –thread 16